Optical fibers and light in biomedical application

7^th Euro Biosensors and Bioelectronics Conference

July 10-11, 2017 Berlin, Germany

Marie Pospisilova

Czech Technical University, Czech Republic

Posters & Accepted Abstracts: J Biosens Bioelectron

Abstract :

Two optical fiber applications for in vivo studies, namely, recording of ciliary beating frequency in respiratory epithelia and analysis of fluorescence spectra from living cells have been studied. First, a method based on a "pigtail" semiconductor laser emitting at 642 nm and using SM and MM fibers has been developed. Backward scattered signals recorded from the pig trachea were evaluated by fast Fourier transform. The detected beating frequency and its temperature dependency are in a good agreement with data obtained with other non-fiber optic devices. This opens the possibility to adapt the fibers for endoscopic studies. Second, absorption and fluorescence spectra were recorded from a â�?�?simplified cellâ�? - a mixture of dominant cell fluorophore: (NADH, pyridoxine and tryptophan) at different concentrations and pH. Calibration curves for NADH concentration were derived and emission spectra, deconvoluted by SW GASPED method, were compared with those of individual pure fluorophore. Spectrometer SHIMADZU UV- 3600 (absorption spectra) and a Fluoromax-4 fluorimeter were used. The same approach was then adopted for cultures of living baker yeasts by using excitation at 340-365 nm. In agreement with literature, the NADH fluorescence (at 460 nm) responded to metabolic conditions imposed. These results indicate that the use of optical fibers may be a valuable tool for studies of cell viability in vivo, for example, in the domain of photodynamic therapy.

Biography :

Email: pospim14@fbmi.cvut.cz