Optimization of inducer concentration in the medium for production of glutaminase from Erwinia

2^nd International Conference and Exhibition on Pharmaceutical Regulatory Affairs

November 23-24, 2012 Hyderabad International Convention Centre, India

Pushpinder Paul

Accepted Abstracts: Pharmaceut Reg Affairs

Abstract :

This purpose of study was to determine effect of inducer concentrations in the medium for production of Glutaminase enzyme from Erwinia. For production of glutaminase enzyme from free cells of Erwinia, various physical and chemical parameters were optimized. The microorganism Erwinia which is a source of glutaminase was procured from Institute of Microbial Technology, Chandigarh and was grown on specified medium. The media was sterilized by autoclaving for 15 minutes at 15 psi/cm2. The microorganism was maintained on agar slants and kept in refrigerator at 4.0 + 0.5 0C for further use and was sub-cultured after every 20 days. The viability check of microorganism was done by gram staining and tryphan blue. The work utilized Beef extract, Peptone, Yeast extract, Sodium chloride and Water as composition of growth medium for the production of glutaminase enzyme. The parameters such as Temp., pH, & Time were optimized. By using these parameters Glutaminase enzyme were produced by adding different concentration of inducers such as. Ammoniumsulphate, Inorganic phosphate & L- Glutamine. It was concluded that as we increase the concentration of inducers, enzyme activity was increases up to a certain concentrations. The enzyme activity was determined by Nessler?s reagent method. So it is cleared from this study that Inducers are very helpful to increase the production of glutaminase enzyme from Erwinia & it was concluded that after adding inducer concentrations Ammoniumsulphate (100mM), Inorganic phosphate (200μL) & L- Glutamine (12.5%) the enzyme production was maximum.

Biography :

Pushpinder paul has completed his Master degree in 2008 from Punjabi University Patiala in Biotechnology at age of 24 years. He is Executive Production at a premier Pharmaceutical organization. He has published 1 research paper at international level and also presented 2 research paper at international level. He has aproximately 5 years of experience in pharmaceutical industry with the production of antibiotics(Penems & Cephalosporins).