Optimizing the enzymatic hydrolysis of cellulose from pretreated waste biomass via response surface methodology

10^th World Congress on Green Chemistry and Technology

July 10-11, 2019 | Paris, France

Luciene S de Carvalho, Leila M A Campos, Heloise O M A Moura and Luiz A M Pontes

Federal University of Rio Grande do Norte (UFRN), Brazil
Salvador University (UNIFACS), Brazil
Federal University of Bahia (UFBA), Brazil

Posters & Accepted Abstracts: J Environ Anal Chem

Abstract :

This work investigated the potential of cellulose isolated from sugarcane bagasse after a combined acid/ peroxide-alkaline (APA) pretreatment, soda/AQ pulping and bleaching steps for producing glucose via enzymatic hydrolysis by using two different enzyme complexes with a full factorial 24 design of experiments (DOE) approach. This research contributes with the accurate statistical assessment of the importance of biomass pretreatment in cellulose digestibility by Celic CTec3 (CT) and Celluclast®1.5 L (CL) enzyme complexes and of how the blend of these two complexes can interfere on the saccharification mechanism. The results showed cellulose and CT factors as having the higher effects on glucose yield; in fact, the response surface methodology (RSM) approach showed that cellulose from pretreated sugarcane bagasse (SBC) presented double the conversion observed for the commercial cellulose (CC), and the CT activity significantly increased when used in conjunction with the CL complex. The design of experiments was able to prove that the morphology and crystallinity of the cellulose feedstock selected for conversion is a factor which has a major effect on glucose production.