Ovarian tissue preservation and cross species transplantation (Marmoset-Monkey to Mouse)

International Conference and Exhibition on Tissue Preservation & Bio-banking

July 20-22, 2015 Barcelona, Spain

R K Chandolia1, V von Schönfeldt2, B Sonntag2 and S Schlatt2

Posters-Accepted Abstracts: J Tissue Sci Eng

Abstract :

Cryopreservation of ovarian tissue has been taken at several levels as it helps in maintaining safe genome of female for production of next generation. For this objective, transplantation of the tissue in a model is needed. Marmoset monkey (Callithrix jacchus) has been advocated an alternative model to large size monkeys. However, there are several challenges to shift to the new model. Cryopreservation of ovarian tissues of the marmoset monkey from adult and young animals have been evaluated using dimethyl sulphoxide (DMSO), 1,2-propanediol (PrOH) or ethyl glycol (EG) using slow freezing protocol. After xenografting in nude mice, the follicles were compared in fresh, cryopreserved and xenografted tissues. When compared to fresh, cryopreservation decreased number of follicles to one third in DMSO, one fifth in PrOH and one sixth in EG. After xenografting in nude mice, the survival of follicles in adult tissue was twice in DMSO group than PrOH group. Prepubertal tissue did not show this difference for primordial follicles, but primary follicles were marginally better in DMSO group. Therefore, for adult marmoset monkey DMSO seems better cryoprotectant for ovarian cryopreservation for xenografting of its tissue. Prepubertal ovarian tissues could survive almost to a similar level in all three cryoprotectants (DMSO, PrOH and EG). This is an indication that the prepubertal tissue has greater tolerance to cryo-injuries. Staining with Proliferating Cell Nuclear Antigen indicated survival of the cells in the xenografted tissue. In human both DMSO and PrOH have been reported equally well but DMSO has been superior in certain aspects.