Sivaperumal Sivaramakrishnan
Bharathidasan University, India
Posters & Accepted Abstracts: Med Chem (Los Angeles)
Chrysin (5,7-Dihydroxy flavone ChR) a natural anticancer bioflavonoid, emerged as a potential drug therapy for almost all types of cancer. Since, ChR was produced from endophytic fungal A. alternate KT380662, isolated from the leaves of Passiflora incarnata L. The ChR production measuring approximately 846 mg L-1 and was confirmed through UV-Vis spectroscopy, FT-IR, LC-ESIMS, and 1H1 NMR analysis. Further, ChR was used as reduction source and capping agent for gold nanoparticles to improve the bioavailability. Nanomaterials are unique size, shape and composition receives much attention on biomedical applications. Herein, a new approach to formulate biofunctionalized metallic gold (ChR-AuNPs) nanoparticles using ChR as a direct bioreductant and capping agent has been used. Particle size and dispersity were controlled through fixing different reaction conditions such as the temperature, pH, concentration of metal ion, stoichiometric proportion of the reaction mixture and incubation time based on their optical properties and SPR effect in UV-visible spectroscopy. The role of hydroxyl and carbonyl groups in functionalizing the metal ions with ChR was confirmed with Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analysis. It was also substantiated that the oxygen group from ChR donates electrons to metal ion and results in complexation; ionic Au(3+) was reduced to Au(0) nano-forms. The physiochemical state of obtained NPs was characterized through different exclusive instrumentation, which shows the presence of highly-stable, spherical, crystalline ChR�AuNPs with an average size of 6-2 nm, respectively. In vitro anticancer results revealed that the formulated ChR�AuNPs exhibit enhanced cytotoxicity over ChR in the treated two different breast carcinoma cell lines (MDA-MB-231 and MDA-MB-468). Further, it was evident that the cell death via the induction of apoptosis. A hemolysis assay with human erythrocytes demonstrates good blood biocompatibility of ChR�AuNPs. Therefore, ChR functionalized metal can be employed as a nano-drug formulation for cancer therapy.
Medicinal Chemistry received 6627 citations as per Google Scholar report
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