Pharmacokinetics study and efficacy evaluation of Ceftriaxone and Tazobactam combination in ESBL E. coli infected diarrhoeic poultry birds

2^nd Indo-Global Summit & Expo on Veterinary

October 26-28, 2015 Hyderabad, India

Tapas Kumar Sar1, Mithin U C1, Tapan Kumar Mandal1, Anjan Bhattacharyya2, Siddartha Narayan Joardar1 and Indranil Samanta1

1West Bengal University of Animal & Fishery Sciences, India 2Bidhan Chandra Krishi Viswavidyalaya, India

Posters-Accepted Abstracts: J Vet Sci Technol

Abstract :

The infections caused by ESBL strains of E. coli were often associated with diarrhea in poultry birds. The present research work was undertaken to study the pharmacokinetic profile of Ceftriaxone and Tazobactam in healthy and ESBL E. Coli infected diarrheic birds (Broiler, Rhode Island Red, Haringhata Black) following single intramuscular dosing of Ceftriaxone Tazobactam combination (8:1) at 28.125 mg kg-1. Efficacy of Ceftriaxone Tazobactam combination was evaluated for ESBL producing E. coli infection in birds (Broiler, Rhode Island Red, Haringhata Black) at pre-determined dosage regimen based on pharmacokinetic study. For induction of infection in the experimental Broiler, Rhode Island Red and Haringhata Black birds, 56����108 CFU/ml of the bacterial culture was inoculated orally. Broilers birds showed severe diarrhea on 7th day following oral inoculation of 1 ml of ESBL producing E. coli whereas moderate diarrhoea was manifested in Rhode Island Red birds from the same day. Due to failure of initial challenge, Haringhata Black birds were again inoculated orally with a higher second dose (112����108 CFU/ml sub culture) after 21 days of first oral inoculation. Ultimately moderate diarrhoea was induced in Haringhata Black birds on 8th day after 2nd inoculation. A single dose of Ceftriaxone Tazobactam combination (8:1) at 28.125 mg kg-1 was administered intramuscularly to healthy and diarrhoeic Broilers, Rhode Island Red and Haringhata Black birds in six groups (Gr BCT & BCT-D, RCT & RCT-D, HCT & HCT-D) each containing six birds and blood samples were collected at pre-determined time intervals. Ceftriaxone and Tazobactam concentrations from plasma were analysed by HPLC to evaluate pharmacokinetic profile. Following induction of diarrhea Ceftriaxone-Tazobactam combination was given two times daily (at 12 hours interval) for three days at 28.125 mg kg-1 intramuscularly. In diarrheic birds of all the three breeds, Ceftriaxone persisted up to 8 hours in presence of Tazobactam and highest plasma concentration was recorded at 0.08 hours. Tazobactam also persisted up to 8 hours while the peak plasma concentration was recorded at 0.25 hour. Longer elimination halflife (t���½ ���²: 3.72���±0.24 hours) associated with higher levels of Tazobactam were evident at 2, 4, 6 and 8 hours in diarrheic Broiler birds in presence of Ceftriaxone. Significantly increased body clearance (ClB: 12.34���±0.58 L kg-1 h-1) of Ceftriaxone in healthy Haringhata Black birds in presence of Tazobactam compared to a mean ClB value of 10.19���±0.57 L kg-1 h-1 in diarrhoeic birds was observed. It was also observed that Tazobactam undergoes rapid absorption in diseased Haringhta Black birds (Ka: 18.77���±1.73 h-1) compared to healthy birds (Ka: 11.91���±1.39 h-1) in presence of Ceftriaxone. Diarrhea began to subside on 2nd day of treatment in all the birds of three groups and a complete recovery was noticed on 3rd day of treatment.

Biography :

Email: tapas.sar@rediffmail.com