Ming Jiang
Sun Yat-sen University, China
Scientific Tracks Abstracts: Metabolomics (Los Angels)
Antibiotic-resistant bacteria become a major threat to the economy and food safety in aquaculture. Although the
antibiotic-dependent strategy is still the mostly adopted option, the development of antibiotic-free approach
is urgently needed to ameliorate the severe situation of the global antibiotic resistance. In the present study, we
showed that modulating the metabolism of zebrafish, Danio rerio, would enhance D. rerio to clear ceftazidimeresistant
Vibrio alginoyticus (Caz-R) in vivo. By generating Caz-R in vitro, we found Caz-R stays longer than
ceftazidime-sensitive V. alginoyticus (Caz-S) in D. rerio, where Caz-R induced less potent immune response than
that of Caz-S. The differential immune response was associated with different metabolism of the host. Through
functional metabolomics, we identified a crucial biomarker, phenylalanine. The abundance of phenylalanine was
increased in both of Caz-S and Caz-R infected hosts but the abundance was higher in Caz-S infected group. This
specific difference indicated phenylalanine could be a metabolite required to clear Caz-R by the host. Exogenous
phenylalanine would enhance the host�??s ability to remove Caz-R, which was through upregulated production of
lysozyme and C3b. Thus, our study demonstrates a novel strategy to boost host�??s immune response to combat against
antibiotic-resistant bacteria.
References:
1. Jiang, M., Gong, Q., Lai, S., Cheng, Z., Chen, Z., Zheng, J., & Peng, B. (2019). Phenylalanine enhances innate
immune response to clear ceftazidime-resistant Vibrio alginolyticus in Danio rerio. Fish & Shellfish Immunology,
912-919.
2. Yang, M., Cheng, Z., Jiang, M., Zeng, Z., Peng, B., Peng, X., & Li, H. (2018). Boosted TCA cycle enhances survival
of zebrafish to Vibrio alginolyticus infection. Virulence, 9(1), 634-644
Ming Jiang is pursuing his Doctor's Degree at Sun Yat-sen University. His current research is focused on the metabolic mechanism of host resistance to drugresistant bacterial infections.
E-mail: jiangm25@mail2.sysu.edu.cn
Metabolomics:Open Access received 895 citations as per Google Scholar report