Phenylalanine modulates innate immune of Danio rerio to clear ceftazidime resistant V. alginolyticus

15^th International Conference on Metabolomics and Systems Biologys

August 29-30, 2019 | Vienna, Austria

Ming Jiang

Sun Yat-sen University, China

Scientific Tracks Abstracts: Metabolomics (Los Angels)

Abstract :

Antibiotic-resistant bacteria become a major threat to the economy and food safety in aquaculture. Although the antibiotic-dependent strategy is still the mostly adopted option, the development of antibiotic-free approach is urgently needed to ameliorate the severe situation of the global antibiotic resistance. In the present study, we showed that modulating the metabolism of zebrafish, Danio rerio, would enhance D. rerio to clear ceftazidimeresistant Vibrio alginoyticus (Caz-R) in vivo. By generating Caz-R in vitro, we found Caz-R stays longer than ceftazidime-sensitive V. alginoyticus (Caz-S) in D. rerio, where Caz-R induced less potent immune response than that of Caz-S. The differential immune response was associated with different metabolism of the host. Through functional metabolomics, we identified a crucial biomarker, phenylalanine. The abundance of phenylalanine was increased in both of Caz-S and Caz-R infected hosts but the abundance was higher in Caz-S infected group. This specific difference indicated phenylalanine could be a metabolite required to clear Caz-R by the host. Exogenous phenylalanine would enhance the hostâ??s ability to remove Caz-R, which was through upregulated production of lysozyme and C3b. Thus, our study demonstrates a novel strategy to boost hostâ??s immune response to combat against antibiotic-resistant bacteria.

References:

1. Jiang, M., Gong, Q., Lai, S., Cheng, Z., Chen, Z., Zheng, J., & Peng, B. (2019). Phenylalanine enhances innate immune response to clear ceftazidime-resistant Vibrio alginolyticus in Danio rerio. Fish & Shellfish Immunology, 912-919.

2. Yang, M., Cheng, Z., Jiang, M., Zeng, Z., Peng, B., Peng, X., & Li, H. (2018). Boosted TCA cycle enhances survival of zebrafish to Vibrio alginolyticus infection. Virulence, 9(1), 634-644

Biography :

Ming Jiang is pursuing his Doctor's Degree at Sun Yat-sen University. His current research is focused on the metabolic mechanism of host resistance to drugresistant bacterial infections.

E-mail: jiangm25@mail2.sysu.edu.cn