Pheophorbide a isolated from Gelidium amansii inhibits adipogenesis by down-regulating adipogenic transcription factors in 3T3-L1 adipocytes

9^th Annual European Pharma Congress

June 26-28, 2017 Madrid, Spain

Ji Sook Han, Mi Hwa Park, Ji Hye Kang, Hak Ju Kim and You Jin Jeon

Pusan National University, Republic of Korea
Silla University, Republic of Korea
Seojin Biotech Co., Ltd., Republic of Korea
Jeju National University, Republic of Korea

Posters & Accepted Abstracts: Pharmaceut Reg Affairs

Abstract :

Background: Adipocyte lipid accumulation causes adipocyte hypertrophy and adipose tissue increment, leading to obesity. Thus, this study investigated the anti-adipogenic effects of pheophorbide A isolated from Gelidium amansii in 3T3-L1 adipocytes. Methods: Upon differentiation of 3T3-L1 pre-adipocytes into adipocytes, they were treated with pheophorbide A (0â��83 �¼M). Results: Pheophorbide A inhibited triglyceride accumulation and stimulated glycerol release in a dose-dependent manner in 3T3- L1 adipocytes. In addition, pheophorbide A significantly decreased leptin levels in 3T3-L1 adipocytes. Pheophorbide A inhibited adipogenesis via suppression of the expression of adipogenic transcriptional factors including peroxisome proliferator-activated receptor �³ (PPAR�³), CCATT/enhancer binding protein �± (C/EBP�±), sterol regulatory element binding protein 1c (SREBP1c), and fatty acid synthase (FAS). It also induced the expression of phosphorylation of AMP-activated protein kinase (AMPK). Conclusion: Pheophorbide A isolated from Gelidium amansii inhibit adipogenesis by down-regulating adipogenic transcription factors in 3T3-L1 adipocytes. These results suggest that pheophorbide A may be useful for the prevention or treatment of obesity owing to its inhibitory effect on adipogenesis.

Biography :

Ji Sook Han is a Professor and is doing research on developing a bioactive compound from natural plants, especially seaweeds, and investigating its effect for the prevention and treatment of obesity and type 2 diabetes. The active compound containing in seaweeds may be a good anti-diabetic source by improving insulin secretary defect or insulin resistance. It may also be a potential anti-obesity source owing to its inhibitory effect on adipogenesis. She evaluates the effect and mechanism of a bioactive compound isolated from natural plant through in vitro and in vivo study.

Email: hanjs@pusan.ac.kr