Phylogenetic diversity, antibiogram and plasmid profiling of enterotoxigenic Klebsiella variicola and Enterobacter species isolated from Iko River-Nigeria

Joint Event on 14^th International Conference on Microbial Interactions & Microbial Ecology & 11^th Edition of International Conference on Advances in Microbiology and Public Health

August 19-20, 2019 Vienna, Austria

Mmuoegbulam Augusta O, Belgini Daiani R B, Iroegbu Christian U, Brisibe Andy, Asikong Etta Bassey E and Oliveira Maia Valeria

University of Calabar, Nigeria
University of Campinas, Brazil
Cross River University of Technology, Nigeria

Scientific Tracks Abstracts: J Med Microb Diagn

Abstract :

Crude oil exploration has led to the presence of pollutants in the marine environment. Among the biota presumably affected by environmental pollution are the microorganisms. It is speculated that oil pollution of the marine environment would create a preponderance of pathogenic and antibiotic resistant survivors which would constitute an enormous public health problem. Such pathogens that could be affected in the event are enterotoxigenic enterobacteria, which abound in Nigerian coastal waters and estuaries. Enterobacteria from Iko River in Nigeria were evaluated for enterotoxigenicity and phylogenetically classified using the 16S rRNA sequencing protocol. The sequence data generated from the PCR amplification and cycle sequencing reaction were matched with available sequences in the ribosomal data project (RDP). Isolates 12A, 13 and EC6 were identified as Klebsiella variicola F2R9T (AJ783916), isolate 11A as Enterobacter ludwigii EN-119T (AJ853891), and isolate 10 as Enterobacter asburiae JCM6051 (AB004744)/cancerogenus LMG 2693T (Z96078). Based on the ligated ileal loop assay, all the isolates were found to be enterotoxigenic with some histopathological effects. The plasmid profile of the isolates was also determined using the Promega protocols; and, with the exception of isolate 13, all the isolates harboured plasmids. Antibiogram showed that the isolates were susceptible to Gentamycin, Ciprofloxacin and Clavulin; however, minimum bactericidal concentration (MBC) determination showed that Gentamycin and Ciprofloxacin were more effective than Clavulin. Evaluation of the relationship between crude oil exposure to presence of plasmid, antibiogram and enterotoxigenicity indicates that exposure to crude oil does not ameliorate or exacerbate antibiotic resistance, enterotoxigenicity and plasmid acquisition.

Biography :

Mmuoegbulam Augusta O is a Lecturer in the Department of Microbiology, University of Calabar, Nigeria. She did her PhD in Molecular Biology research internship in CPQBA, University of Campinas, Brazil under TETFund sponsorship. Her masters and PhD specializations were Medical Microbiology and Pathogenic Microbiology/Public Health respectively. Her research interests include pathogenic microbiology, public health, molecular biology, bacteriology, antibiotic resistance, infectious diseases, virology, immunology, genomics and cancer therapy.

E-mail: oluchidinma@yahoo.com