Xinfen Gao, Marie Fougere-Danezan, Zhangming Zhu and Yundong Gao
Chinese Academy of Sciences, China
Posters-Accepted Abstracts: J Mol Genet Med
There are about 150-200 species of the genus Rosa L which is widely distributed in the northern hemisphere. We have been studying this genus in following three aspects. The first is the phylogenetic study of Rosa. We analyzed the sequences data from the nrDNA ITS region and GAPDH gene, the plastid psbA-trnH spacer, trnL intron, trnL-F spacer, trnS-G spacer and trnG intron, the results showed molecular trees are not accordance with the species tree based on the morphological data. The ancestral area reconstruction suggests that despite an early presence on the American continent, most extant American species are the results of a later re-colonization from Asia, probably through the Bering Land Bridge. The results suggest more recent exchanges between Asia and western North America than with eastern North America. Secondly, we explored the hybrid origin of some species by both nrDNA and cpDNA fragments confirmed the wild rose hybrid occurred not only between species of the same section but also between the different sections. The third is studying the origin and differentiation of species complex, gene flow between populations and gene introgression. In the present study, Rosa sericea complex as an example, we detected the chloroplast DNA trnL-trnF, ndhF-rpl32 and ndhJ-trnF and nuclear microsatellite (nSSR) on eight sites from 763 individuals of 62 populations preliminarily obtained 42 haplotypes. The result reveals the evolutionary lineage of this complex, the demographic history of R. sericea which diverged in the middle Pleistocene, was mostly affected by climatic oscillations instead of by geographical barriers.
Email: xfgao@cib.ac.cn
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