Physiological stress results in potent suppression of inkt cells following alpha-galactosylceramide mediated activation

International Congress on Neuroimmunology and Therapeutics

DoubleTree by Hilton Hotel San Francisco Airport, San Francisco, CA, USA

Chantana Boonyarat1,2, Pornthip Waiwut1, Chavi yenjai1, Jiranan Chotitumnavee1

Scientific Tracks Abstracts: J Neurol Disord

Abstract :

Beta-amyloid (Aβ) is a major pathogenic peptide for Alzheimerâ??s disease (AD). The Aβ monomers aggregate into oligomeric and fibrillar forms which have been implicated as the toxic species inducing the neuronal dysfunction. Chalcone is known for its anti-oxidant and anti-inflammatory functions. Therefore, we investigated the effects of five chalcones extracted from Dorris intica on aggregation of Aβ peptides and neuroprotection. Five chalcones extracted from the fruit of Dorris intica included obovatachalcone, tunicatachalcone, ovalichalcone, pongamol, derrischalcone. The results exhibited that almost compounds except pongamol showed an ability to inhibit Aβ1-42 aggregation assessed by Thioflvin T assay. Ovalichalcone exhibited the highest activity with inhibitory percentage of 75.1 at 50 μM. Molecular modeling studies revealed that these compounds interacted with Aβ1-42 side chain at salt bridge (Asp23 â?? Lys28) and hydrophobic region (residue 17 - 21) which are important for amyloid aggregation. For neuroprotection assessed by cell culture model, our results showed that all of these compounds could reduce neuronal death induced by Aβ1-42 toxicity. The overall results indicated that the chalcones extracted from Dorris intica possess amyloid aggregation inhibitory action and neuroprotection. Thus, these compounds can be considered as a promising candidate for further pharmacological development in Alzheimerâ??s therapy.

Biography :

Chantana Boonyarat has completed her PhD from Mahidol University, Bangkok, Thailand. She is the lecturer at the faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand. She has published more than 15 papers in reputed journals.