Ashraf Hamed
Minya University, Egypt
Posters & Accepted Abstracts: Altern Integr Med
From the phytochemical study of the Markhamia platycalyx (Baker) Sprague leaves, twelve compounds were isolated viz., phytol (1), n-octacosanoic acid (2), tormentic acid (8) and �²-sitosterol-3-O-(6'-O-heptadecanoyl)-�²-D-glucopyranoside (10) were isolated for the first time in the genus Markhamia. Additionally, six compounds were reported for the first time in the species viz., �²-sitosterol (3), ursolic acid (5), oleanolic acids (6), pomolic acid (7), 2-epi-tormentic (9), �²-sitosterol-3-O�²-D-glucopyranoside (11) and Finally, two more compounds were previously reported; stigmasterol (4) and acteoside (12). The structure elucidation was based on comparison of their chemical, physical, chromatographic properties, spectral data (MS and NMR) with literature in addition to direct comparison with authentic compounds. The antimicrobial and antiprotozoal activities of the total ethanolic extract (TEE), its fractions as well as the isolated compounds were tested. The TEE was the most effective exhibiting the lowest MIC (1.0 �µg/mL) against E. coli. Moreover, ursolic acid, acteoside and epi-tormentic acid were the most potent against P. aeruginosa with MICs 1.2 �µg/mL, 1.6 �µg/mL and 2.3 �µg/mL, respectively. Additionally, the epi-tormentic acid and acteoside showed the lowest MICs (1.2 �µg/mL) against Candida glabrata. All the above results are higher than standard drugs. While, the secondary phase assay of the petroleum ether fraction revealed IC50 of 26760 ng/mL against P. falciparum D6 and 22430 ng/mL against P. falciparum W2. Additionally, the dichloromethane fraction was the most active against L. donovani amastigotes in THP1 with 86% inhibition. Finally, the epi-tormentic acid and �²-sitosterol-3-O-�²-Dglucopyranoside did not exhibit any effect on hepatitis C virus.
Alternative & Integrative Medicine received 476 citations as per Google Scholar report
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