Possible immunochemical alterations in the plasma levels of TNF-α and IL-10 in the traditional application of Vernonia amygdalina (Ewuro) leaves in the treatment of Diabetes mellitus

Joint Event on 2^nd International Conference on Medical and Health Science & 8^th Annual Congress and Medicare Expo on Primary Healthcare

July 26-27, 2019 Melbourne, Australia

Mathew Folaranmi Olaniyan, Alade Aderinto Ogunlade and David Atere

Edo University Iyamho, Nigeria
Achievers University, Nigeria

Posters & Accepted Abstracts: J Health Edu Res Dev

Abstract :

Introduction & Objective: Diabetes mellitus can induce release of free radicals and oxidative stress which can trigger production of cytokines. Vernonia amygdalina has anti-diabetic activity due to its phytochemical constituents. The study objective was designed to determine the possible immunochemical alterations in the plasma levels of TNF- α and IL-10 in the traditional application of Vernonia amygdalina (Ewuro) leaves in the treatment of diabetes mellitus.

Method: The study populations include all 33 Diabetes mellitus patients (51â??67 years; male-21; female-12) who had not commenced any form of medication or treatment and received herbal treatment in 10 herbal homes of Saki-West local government area in Nigeria. 27 age-matched volunteers who were treated on insulin medication in the hospital almost within the same period were also investigated. Patients who were positive to Acid Fast Bacilli Sputum test, Plasmodium spp., identification, HBsAg, anti-HCV and HIV-1 p24 assays were not included. Plasma TNF- α, IL-10, HBsAg, anti-HCV and HIV- 1 p24 were determined in the patients by ELISA while identification of Acid Fast Bacilli and Plasmodium spp., were carried out by Ziehl-Neelsen and Giemsa -Thick blood film staining respectively.

Results: There was a significant decrease in the plasma levels of TNF-α , IL-10 and glucose in diabetes mellitus patients after the administration of raw liquid extract of Vernonia amygdalina and insulin compared to their basal samples before the commencement of the treatment (p<0.05).

Conclusion: The study revealed a significant increase in plasma TNF-α, IL-10 and glucose in diabetes patients which returned to normal plasma values after treatment using raw liquid extract of Vernonia amygdalina leaves and insulin.

Biography :

E-mail: olaniyanmat@yahoo.com

olaniyanmat@gmail.com