Post-PCR DNA modification using metal-free click chemistry and its applications in cancer cell recognition

5^th World Congress on Cancer Therapy

September 28-30, 2015 Atlanta, USA

Ke Wang, Kaili Ji, Danzhu Wang, Weixuan Chen, Yueqin Zheng, Chaofeng Dai, and Binghe Wang

Department of Chemistry and Center for Diagnostics and Therapeutics, Georgia State University, USA

Posters-Accepted Abstracts: J Cancer Sci Ther

Abstract :

DNA molecules endowed with extra functional groups are very useful in a wide variety of applications including sensing, developing new therapeutics and in vitro aptamer selection. Conceivably, such DNA modifications can be achieved by first incorporating a functional group handle, which allows further manipulations, on a natural nucleotide base and then conduct synthesis using either chemical methods or a DNA polymerase. In the latter case, it requires that a sidechain handle be tolerated by the DNA polymerase used. Herein we describe polymerase-mediated incorporation of trans-cyclooctene modified thymidine triphosphate. Subsequently, the trans-cyclooctene group was reacted with a tetrazine tethered to other functional groups through a fast copper-free click reaction. The utility of this DNA functionalization method was demonstrated with the incorporation of a boronic acid group and a fluorophore. Boronic acid modified DNA molecules were further applied in aptamer selection targeting cancer cells.

Biography :

Email: kwang10@gsu.edu