Prevalence of Beta-Papilloma virus in early onset squamous cell skin cancer

5^th Asia-Pacific Summit on Cancer Therapy

July 20-22, 2015 Brisbane, Australia

Gayathri St George, Maurits N.C. de Koning, Robyn Dalziell, Mariet Feltkamp, Wim G.V. Quint and Graham J Mann

Posters Accepted Abstracts: J Cancer Sci Ther

Abstract :

Background and objectives: Cutaneous squamous cell carcinoma (cSCC) are frequently diagnosed and account for most of the 410 deaths that occur due to non-melanoma skin cancer in Australia each year. Evidence is mounting that HPV also contributes to cSCC. The objective of this study was to test this hypothesis further by determining the betaPV distribution in Australian cSCC cases diagnosed at a relatively early age, using both related and population controls. Methods: Cases (n=111), recruited from sequential records of a dermatopathology referral service in Sydney, had been diagnosed with cSCC of any stage <50 yr (<10% of cases in Australia are diagnosed <50yr). The cases nominated both sibling (n=53) and unrelated partner (n=66) controls. Eyebrow hair bulbs were collected for detection and genotyping of betaPV using the PM-PCR reverse hybridization assay (RHA) method. Results: Results from half the sample show an overall carriage frequency of 76% in cases, 68% in sibling controls and 72% in unrelated controls (p=0.018). HPV types 5, 15, 23, 24, 38 were the most commonly recurring, each present in >25% of subjects. 14% of the subjects tested negative for each of the 25 papillomavirus types, with no association with case control status. Four or more types were present in 35% of cases, 16% of siblings and 31% of unrelated controls (p=0.336). Putatively high cancer risk types (5, 8, 15, 20, 24, 36, 38) were present in 62% of cases, 48% of sibling controls and 57% of unrelated controls; 17% of the cases and unrelated controls, but 2% of the siblings, carried three or more types from the high-risk group. Conclusion: The data suggest that control siblings were less likely than case siblings to exhibit long-term carriage of (multiple) betaPV types. However, there is no evidence at this stage for association of betaPV with early-onset cSCC, especially when cases were compared with unrelated controls.