Prevalence of biofilms and enterotoxins produced by Staphylococcus aureus-inducing pneumonia in South-South geopolitical zone Nigeria

JOINT EVENT ON 15^th International PHARMACEUTICAL MICROBIOLOGY AND BIOTECHNOLOGY CONFERENCE & 10^th Annual MEDICAL MICROBIOLOGY SUMMIT & EXPO

June 21-23, 2017 London, UK

Enwa Felix Oghenemaro

Delta State University, Nigeria

Posters & Accepted Abstracts: J Med Microb Diagn

Abstract :

The emergence of recalcitrant strains of Staphylococcus aureus is also alarming and an awareness of the virulence has been shown to help prevent, combat or eradicate Staphylococcus aureus infections. This study aimed at evaluating the biofilm forming capacity of Staphylococcus aureus, prevalence of Staphylococcus aureus induced biofilms and enterotoxins as well as prevalence of Staphylococcus aureus induced pneumonia in south-south geopolitical zone, Nigeria. A total of 1500 clinical specimens (sputa) were collected from clinically diagnosed pneumonia patients in randomly selected health institutions in south-south geopolitical zone, Nigeria and cultured using selective medium for Staphylococci. Seventy nine (79) samples out of 1500 investigated yielded Staphylococcus aureus. The 79 clinical isolates were further screened for biofilm formation using crystal violet binding assay and for enterotoxins using reverse passive latex agglutination (RPLA) method. The results showed that prevalence of Staphylococcus aureus induced pneumonia in South-South geopolitical zone, Nigeria is low; but the biofilm forming capacity of Staphylococcus aureus is high with the highest and lowest mean biofilm thickness (absorbance) of 0.358�±0.06 and 0.211�±0.07, respectively. Also the results showed that the most prevalent Staphylococcus aureus enterotoxins in south-south geopolitical zone, Nigeria are enterotoxin B. These findings are very important in monitoring the virulence and resistance patterns of Staphylococcus aureus.

Biography :

Email: felixenwa@delsu.edu.ng