Process Raman spectroscopy for in-line monitoring of mammalian cell cultures in real time

4^th International Conference and Exhibition on Biologics & Biosimilars

October 26-28, 2015 Baltimore, USA

Alexander Pitters

Kaiser Optical Systems, Inc., France

Posters-Accepted Abstracts: J Bioanal Biomed

Abstract :

Mammalian cell cultures are complex processes where cells are cultivated under highly controlled conditions using media with a very high number of components. Current effort is focused on obtaining a better understanding of mammalian cell cultures by cultivating predominantly CHO cells for therapeutic protein production. To ensure a healthy progression of the cell culture, it is important to understand and monitor the stages of the biologic manufacturing. In order to build quality into a process a primary step is to analyze the process, understand what the critical quality attributes are, and monitor or rather control those factors. Consequently, there is a significant interest and value in techniques that provide instantaneous response for monitoring and analyzing biopharmaceutical processes. Molecular techniques - such as Raman spectroscopy are widely used for PAT applications, because they provide in-situ information in real-time. Real-time measurements within Bio-pharma are achieved for Glucose, Glutamine, Glutamate, Lactate, Ammonium, Viable Cell Density, Total Cell Density, Osmolality, Monoclonal Antibodies and Viability. The analyzer software enables a fully integrated bioprocess management and the instruments allow controlling 4 bioreactors from a working distance of 1 to 300 m. Raman spectroscopy is compatible with aqueous systems and spectra contain both chemical and physical information. Analyzers can be used to study solids, liquids or gas without sampling accessories or preparation. Kaiser Optical Systems is the leader in Raman instrumentation and advanced holographic components for spectroscopy. Products and services are positioned in pharmaceutical and chemical manufacturing around the world.

Biography :

Email: pitters@kosi.com