Protective effect of Euphorbia neriifolia leaves and its isolated flavonoid against N-nitrosodiethylamineinduced hepato carcinoma in mice

5^th Asia-Pacific Summit on Cancer Therapy

July 20-22, 2015 Brisbane, Australia

Pracheta Janmeda

Posters Accepted Abstracts: J Cancer Sci Ther

Abstract :

Protective effect of hydro-ethanolic extract of Euphorbia neriifolia (EN) leaves and an isolated flavonoid (ENF) was investigated against N-Nitrosodiethylamine (DENA)-induced hepatocarcinoma in mice. Experimental mice were pretreated with 150 and 400 mg/kg body wt of EN, 0.5% and 1% mg/kg body wt of butylated hydroxylanisole (BHA) as a standard antioxidant and 50 mg/kg body wt of ENF for 21 days prior to the administration of a single dose of 50 mg/kg body wt of DENA. Levels of liver markers alanine amino-transferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), lipid peroxidation (LPO) and antioxidants superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST) and reduced glutathione (GSH) were measured to determine the hepato carcinoma caused by DENA. Activities of liver markers and antioxidants were significantly decreased (p<0.001) while LPO level was significantly (p<0.001) increased after DENA administration as compared with the normal control group (p<0.001). Pretreatment with EN and ENF counteracted DENA-induced oxidative stress (LPO) and exerted its preventive effects by restoring the levels of liver markers (AST, ALT and ALP) and antioxidants (SOD, CAT, GST and GSH) in liver tissue. In conclusion, the present study showed significant anticarcinogenic potential of the hydro-ethanolic extract EN and ENF against DENA induced hepatic carcinogenicity.