Protective effect of histidine on para-nonylphenol enhances 1-methyl-4-phenylpyridinium ioninduced hydroxyl free radical generation in rat striatum

3^rd International Conference on Central Nervous System Disorders and Therapeutics

October 02-03, 2017 Vienna, Austria

Toshio Obata

Osaka Aoyama University, Japan

Posters & Accepted Abstracts: J Neurol Disord

Abstract :

The present study examined the antioxidant effect of histidine, a singlet oxygen (1O2) scavenger, on para-nonylphenol, an environmental estrogen-like chemical, enhances 1-methyl-4-phenylpyridinium ion (MPP+)-induced hydroxyl radical (â�?¢OH) generation in extracellular fluid of rat striatum. Rats were anesthetized, and sodium salicylate in Ringer's solution (0.5 nmol/l/min) was infused through a microdialysis probe to detect the generation of â�?¢OH as reflected by the non-enzymatic formation of 2,3-dihydroxybenzoic acid (DHBA) in the striatum. Induction of para-nonylphenol (10 M) significantly enhanced MPP+-induced â�?¢OH generation. However, histidine (25 mM) decreased the para-nonylphenol-induced â�?¢OH formation. Although the level of MPP+-induced â�?¢OH formation trapped as DHBA after para-nonylphenol treatment increased, para-nonylphenol failed to increase either the level of dopamine (DA) and DHBA formation in the reserpinized anuimals. When iron (II) was administered to para-nonylphenol (10 M)-pretreated rats, iron (II) clearly produced a dosedependent increase in â�?¢OH formation, compared with MPP+-only treated animals, that showed a positive linear correlation between iron (II) and DHBA (R2=0.983) in the dialysate. However, in the presence of histidine (25 mM), small increase in the level of DHBA products were observed. These results indicate that para-nonylphenol enhanced â�?¢OH generation on 1O2 production, and histidine may have preventive effect on para-nonylphenol and MPP+-induced â�?¢OH generation in rat striatum.

Biography :

Toshio Obata is currently working as a Professor in the Department of Nursing at Faculty of Health Sciences, Osaka Aoyama University, Japan. He has published several original research papers in the reputed journals and also participated into several scientific meetings.