Protein expression profiling of sorafenib-resistant human renal cancer stem-like cells in three-dimensional in vitro model

JOINT EVENT:19^th Euro Congress on Cancer Science and Therapy & 25^th Cancer Nursing & Nurse Practitioners Conference

July 17-19, 2017 Lisbon, Portugal

Zofia F Bielecka, Agata Malinowska, PaweÃ?Â? Krasowski, Claudine Kieda and Cezary Szczylik

Military Institute of Medicine, Poland
Warsaw Medical University, Poland
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Poland
Centre for Molecular Biophysics, Cell Recognition and Glycobiology, France

Scientific Tracks Abstracts: J Cancer Sci Ther

Abstract :

Primary-like resistance phenomenon to sorafenib (TKI) was previously observed in hypoxic conditions in two 3D models as well as in one 2D model in HKCSCs (human kidney cancer stem-like cells) cell line (Celprogen, cat no. 36117-44). Herein, we investigated the molecular background behind this phenomenon in 12 hypoxic total protein samples (each in triplicate) of renal cancer cells: primary HKCSCs resistant to sorafenib in hypoxia and other cell lines sensitive to sorafenib treatment in the same conditions: parental HKCSCs cell line, primary and metastatic renal cell carcinoma (RCC) cells from Memorial Sloan Kettering Cancer Centre (SK-RC-44 and SK-RC-45), metastatic RCC cell line (pleural effusion) ACHN cell line and HEK-293 cell line as a control. Additional control condition was untreated adequate cell lines in hypoxia. The acquired MS/MS data were pre-processed with Mascot Distiller software (v. 2.5, Matrix Science), and a database search was performed using the Mascot Search Engine (Matrix Science, Mascot Server 2.4.1) against the Swiss-Prot database restricted to human proteins. The results revealed potential candidates as elements of molecular mechanisms of primary resistance to sorafenib in renal cell carcinoma; those results will be further confirmed using Western blot analysis, possible confirming that apart from impact of hypoxia, the addition of targeted therapies alter signaling pathways in renal cancer cells, which means that mechanisms other than those involving hypoxia-inducible factors (HIFs) may be responsible for TKI-resistance.

Biography :

Zofia F Bielecka is a Medical Biotechnologist, currently working on tyrosine kinase inhibitors resistance in renal cell carcinoma. She is a PhD student at Warsaw Medical University, Poland (International School of Molecular Medicine). She is also a Research Assistant at Military Institute of Medicine in Warsaw, Poland and works in Laboratory of Molecular Oncology. She has gained considerable experience at the University of York, England and at the Lund University, Sweden. She has published 7 papers in reputed journals and has been serving a Reviewer in Elsevier journals. She is also a co-author of one patent application.

Email: zofia.bielecka@gmail.com