Role of insulin-like growth factor-1 (IGF-1) in IgA-mediated nephropathy: Ex vivo studies on rat glomerular mesangial cells

8^th World Nephrology Conference

August 15-16, 2016 Sao Paulo, Brazil

Amal A Al-Eisa and Gursev S Dhaunsi

Kuwait University, Kuwait

Posters & Accepted Abstracts: J Nephrol Ther

Abstract :

Background: IgA nephropathy (IgAN) is a common renal disorder among children worldwide, including Kuwait. Glomerulonephritis due to mesangial proliferation is responsible for renal dysfunction in IgAN, however molecular mechanisms of pathogenesis are not well known. In this study, we explore the role of insulin-like growth factor-1 (IGF-1), a potent mitogen with vital role in growth and development of children, in pathogenesis of IgAN using cultures of glomerular mesangial cells (GMC). Methods: GMC were isolated from rat kidneys using sieving and enzymatic digestion of tissue homogenates, and cultured in RPMI 1640 medium. GMC culture was treated with IgA (0-10 ug.ml) in the presence or absence of IGF-1, platelet-derived growth factor (PDGF), epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF). Results: Treatment of GMC with IgA (5 -10 ug) significantly increased the BrdU incorporation in a serum/mitogen-free medium and also markedly potentiated IGF-1-/PDGF-induced DNA synthesis. IgA was observed to significantly increase the levels of IGF-1 in culture supernatants with no marked effect on PDGF content. Conclusion: These findings show that IgA enhances the IGF-1 activity in GMC and suggest a role for IGF-1 in pathogenesis of IgAN.

Biography :

Email: amal@hsc.edu.kw