Screening of antioxidant and anti-acetylcholinesterase activities of plants from Malpighiaceae family

International Conference and Exhibition on Marine Drugs and Natural Products

July 25-27, 2016 Melbourne, Australia

Karina Fraige, Rodrigo S Cabral, Maria Claudia Marx Young and Vanderlan S Bolzani

Sao Paulo State University, Brazil
Botanical Institute, S�£o Paulo, Brazil

Posters & Accepted Abstracts: J Pharmacogn Nat Prod

Abstract :

The use of plants in traditional medicine comes from antiquity, and secondary metabolites such as alkaloids, flavonoids and tannins can be responsible for several therapeutic properties. Antioxidant compounds from plants can scavenge free radicals, playing an important role in the prevention of oxidative stress, responsible for many degenerative diseases, including Alzheimerâ��s disease (AD). The compounds currently used to treat AD are reported to have many adverse effects, so the search for new compounds from natural sources is required. The tropical family of Malpighiaceae comprises 65 genera and about 1,250 species, with 32 genera and 300 species occurring in Brazil. This family is known by its antimicrobial activity, commonly related to triterpenes, alkaloids and phenolic compounds. The present research involves the evaluation of the antioxidant capacity by DPPH�� and ABTS+�� assays of plants from Malpighiaceae family (Byrsonima, Banisteriopsis, and Stigmaphyllon). The extracts were prepared with solvents of increasing polarity (hexane, ethyl acetate and methanol). All extracts were subjected to acetylcholinesterase inhibition assay by thin layer chromatography. The methanol extracts were subjected to the antioxidant assays and HPLC-DAD analysis in a C18 column. The methanolic extracts of Byrsonima species showed high antioxidant activity (EC50 from 9.4 to 11.5 �¼g/mL), probably due to the composition of flavonoids and tannins, whereas the other species presented low or moderate activity. The preliminary screening of acetylcholinesterase assay showed that the studied species exhibited a weak activity on the inhibition of this enzyme related to one or a group of compounds eluting in the same retention factor.

Biography :

Email: kfraige@yahoo.com.br