Screening of exon 11 for BRCA1 and BRCA2 using the high resolution melting in Moroccan breast cancer patients for diagnosis

5^th Asia-Pacific Summit on Cancer Therapy

July 20-22, 2015 Brisbane, Australia

Meryam El Khachibi1, Brehima diakite1, Abdallah Badou1, Mohamed Amine Senhaji1, Hassan Jouhadi2, Abdelhamid Barakat3, Abdellatif Benieder2 and Sellama Nadifi1

Posters Accepted Abstracts: J Cancer Sci Ther

Abstract :

Introduction: Identification of specific mutation targets in cancer may lead to discovery of the genes modulating cancer susceptibility and/or prognosis. BRCA1 and BRCA2 is the most studied in association with Breast cancer. We evaluated the use of high-resolution melting (HRM) to screen for mutations in those genes in Moroccan patients and assess their clinical significance. Material & Methods: The HRM analysis was used to screen coding exons from 71 breast cancer patients. All sequence variants detected by HRM resulted in abnormal shape of the melting curves. The identified mutations and known single nucleotide polymorphisms (SNP) were subsequently confirmed by sequencing and distribution of the SNP genotypes was determined by SNaPshot analysis. Results: We identified specific missense mutations in different breast cancer Moroccan patients. For the exon 11 in BRCA1 gene, we first used two samples with previously known mutations, �??2924delA and 3398delC�?� that yielded atypical shape in melting curves relative to wildtype control sequences and for two patients we observed an atypical curve which we sequenced using the conventional Sanger approach and we confirmed the presence of the same SNP (c.2612C>T) in both samples. Regarding the exon 11 of BRCA2 we detect for three samples an atypical curve which we sequenced after by Sanger Approach and we identified the presence of the same SNP c.6513G>C. Conclusions: The HRM analysis represents a reliable and highly sensitive method for mutation scanning of multiple exons.