Searching of potential lung cancer biomarkers in bronchoalveolar lavage fluid using metabolomic approaches

10^th Global Annual Oncologists Meeting

July 11-13, 2016 Cologne, Germany

Gomez-Ariza J L, Callejon-Leblic B, Garcia-Barrera T, Gravalos-Guzman J and Pereira-Vega A

University of Huelva, Spain
Hospital Juan Ramon Jimenez, Spain

Posters & Accepted Abstracts: J Cancer Sci Ther

Abstract :

Lung cancer (LC) is one of the 10 most common causes of death in the World and the search for biomarkers in biological fluids, for early diagnosis of this disease is a very challenging task. Metabolomics of bronchoalveolar lavage fluid (BALF) provides information on cellular and biochemical epithelial surface of the lower respiratory tract yielding about 1 ml of pulmonary secretions. Metabolomic studies have not been previously performed with BALF samples from lung cancer patients for diagnosis purposes. In this study, two complementary metabolomic techniques based on direct infusion high resolution mass spectrometry (DI-ESI-QTOF-MS) and gas chromatography mass spectrometry (GC-MS) have been applied for the first time to establish statistically significant differences between LC and non-cancer controls (C), using partial least square discriminant analysis (PLS-DA) to identify potential biomarkers of lung cancer. A total of 43 altered metabolites were found in BALF from LC including urea, acetamide, palmitic acid, stearic acid, adenine and phosphocholine, which are overexpressed, while other 36 metabolites are underexpressed. Finally, biomarkers specificity and sensitivity was established according to the area under the curve of the receiver operator characteristic (ROC curves), which could be used to distinguish patients with lung cancer from control subjects. The pathway analysis indicated that glycerophospholipid metabolism was at the top altered pathway in this disease.

Biography :

Email: ariza@uhu.es