Serum uric acid is effectively correlated with the quality of donated red blood cells under blood bank conditions

25^th WORLD CANCER CONFERENCE

October 19-21, 2017 | Rome, Italy

Vasileios L Tzounakas, Dimitrios G Karadimas, Alkmini T Anastasiadi, Hara T Georgatzakou, Eleftheria Kazepidou, Dimitris Moschovas, Athanassios D Velentzas, Anastasios G. Kriebardis, Nikolaos E. Zafeiropoulos, Apostolos Avgeropoulos, Marilena Lekka, Konstantinos E Stamoulis, Issidora S Papassideri, and Marianna H Antonelou

National & Kapodistrian University of Athens (NKUA), Greece
Department of Chemistry, University of Ioannina
Department of Materials Science Engineering, University of Ioannina
Technological and Educational Institute of Athens
Hellenic National Blood Center, Acharnes, Greece

Posters & Accepted Abstracts: J Cancer Sci Ther

Abstract :

Statement of the Problem: During their preservation at blood banks, red blood cells (RBCs) undergo several physiological alterations/deteriorations collectively known as â�?�?RBC storage lesionâ�?. A significant part of the storage lesion is driven by oxidative stress, while some of its critical aspects are considered donor-related. Having in mind that serum uric acid (UA) represents almost 60% of the total antioxidant capacity of the donorâ�?�?s plasma, the aim of this study was to provide evidence regarding the potential usefulness of UA as a donor-specific marker of storage quality. Methodology & Theoretical Orientation: For this purpose, 47 non-leukoreduced units of RBC concentrates in CPDA1 produced from male regular blood donors were stored for 35 days. Several storage quality parameters (cell shape, redox homeostasis, extracellular vesicles/EVs etc.) were examined at the beginning (day 2), the middle (day 18) and the end (day 35) of the storage period. SPSS was used for statistical analysis. Findings: Antioxidant capacity of the blood bagsâ�?�? supernatant was correlated with the UA levels i n v i v o (R=0.718, p<10-7) throughout the storage period. A posteriori splitting of the donors in high- and low-UA groups, revealed statistically lower intracellular ROS and calcium accumulation after the middle of storage (p<0.05) in the high UA group. Finally, units from high UA donors demonstrated lower levels of irreversibly modified RBCs (22.5�?±2.9% vs 27.1�?±1.6%) and different size distribution of EVs on day 35 of storage (p<0.05). Conclusion & Significance: Variability in UA levels in vivo is maintained during storage and of note, it seems to be associated with the redox status and morphology of stored RBCs. Uric acid as a donorâ�?�?s signature in blood components may be a very promising candidate biomarker of RBC storage lesion. This study was supported by â�?�?IKY FELLOWSHIPS OF EXCELLENCE FOR POSTGRADUATE STUDIES IN GREECE â�?�? SIEMENS PROGRAMâ�? to Vasileios Tzounakas.

Biography :

Vasileios L Tzounakas is a Post-doctoral researcher at the Department of Biology (Section of Cell Biology & Biophysics) of the National and Kapodistrian University of Athens (NKUA). He has obtained Ph.D. in Cell Biology. He has served as reviewer in international journals while his main research interests include blood transfusion biology (mainly, red blood cell storage lesion in blood products used for transfusion), erythrocyte biology in health and disease and the study of extracellular vesicles. He has expertise in evaluating the key parameters that affect storage lesion and posttransfusion performance of red blood cells and in the management of blood supplies in a way that will lead to the individualization of transfusion therapy. In this context, he has focused on the elucidation of storage lesion’s features that may serve as a donor’s signature, namely “the donor variation effect.