Simple and versatile synthetic polydopamine-based surface supports reprogramming of human somatic cells and long-term self-renewal of human pluripotent stem cells under defined conditions

6^th International Conference and Exhibition on Materials Science and Engineering

September 12-14, 2016 Atlanta, USA

Ping Zhou

Peking University, China

Posters & Accepted Abstracts: J Material Sci Eng

Abstract :

Human pluripotent stem cells (hPSCs) possess great value in the aspect of cellular therapies due to its self-renewal and potential to differentiate into all somatic cell types. A few defined synthetic surfaces such as polymers and adhesive biological materials conjugated substrata were established for the self-renewal of hPSCs. However, none of them was effective in the generation of human induced pluripotent stem cells (hiPSCs) and long-term maintenance of multiple hPSCs, and most of them required complicated manufacturing processes. Polydopamine (PDA) has good biocompatiblity, is able to form a stable film on nearly all solid substrates surface, and can immobilize adhesive biomolecules. In our study, carboxymethyl chitosan was used as a linker to orthogonally and controllably attach adhensive peptide to PDA coated cell culture plates for the culture of hPSCs. This synthetic surface was demonstrated that not only support the reprogramming of human somatic cells into hiPSCs under defined conditions, but also sustain the growth of hiPSCs on diverse substrates. Moreover, the proliferation and pluripotency of hPSCs cultured on the surface were comparable to Matrigel for more than 20 passages. Besides, hPSCs were able to differentiate to cardiomyocytes and neural cells on the surface. This polydopamine-based synthetic surface represents a chemically-defined surface extensively applicable both forfundamental research and cell therapies of hPSCs..

Biography :

Email: zhp07@pku.edu.cn