Simple, rapid and valid thin layer chromatographic method for determining malachite green residues in fish

European Chemistry Congress

June 16-18, 2016 Rome, Italy

Cecile Soulignac

University of Rouen, France

Posters & Accepted Abstracts: Chem Sci J

Abstract :

A thin-layer chromatography (TLC) method for determining the sum of MG and LMG residues in fish is described. The total MG on TLC gave a characteristic green color at visible light by naked eyes. This property was used to determine TCs on silica gel by densitometer. Using a mobile phase of ACN: acidified MeOH: acetic acid 0.1N (8:1:1, v/v/v) and a silica gel thinlayer, total MG were well separated from impurities. The total MG was selectively determined by densitometry at 620 nm. The relative standard deviation for the method at 1�µg kg-1 was <3.0%. The detection capability (CC�²) was 1 �µg kg-1 and the limit of detection (LOD) was 0.5 �µg kg-1. Trueness (recovery) was 73.4%. The variation of results was not significant, as the coefficient of variation (CV%) was 8.71% (<20%). cecile.soulignac@etu.univ-rouen.fr