Single cell RNA-seq and its application in lung development and maturation

6^th International Conference on Genomics & Pharmacogenomics

September 12-14, 2016 Berlin, Germany

Yan Xu

University of Cincinnati, USA

Posters & Accepted Abstracts: J Mol Genet Med

Abstract :

Genetic and phenotypic heterogeneity among cells is a general phenomenon; such heterogeneity is associated with development, dynamics, function and dysfunction of tissues. An important challenge to the research society is to understand the genetic regulatory programs driving individual cell structural and functional differentiation of the diverse cell types. Recent studies using single cell transcriptome analysis illustrates the power to measure and understand cellular heterogeneity in complex biological systems. Single cell transcriptome analysis provides the opportunity to link gene expression network with the physiology, function and phenotype of every individual cell. To facilitate the basic and clinic research applications of single cell genomics, we have developed analytic pipelines and web tools for single cell transcriptome analysis. We have successfully utilized the analytic methods to identify cell specific gene expression patterns underlying normal lung developmental processes. We identified major cell types and the mRNA signatures in single cells isolated from mouse lung at different embryonic stages, developed cell specific transcriptional regulatory network and identified major driving forces at critical stages of lung cell differentiation and maturation. A thorough understanding of the cells and gene expression driving normal lung maturation will promote the understanding of the pathogeneses of lung diseases in both infants and children.

Biography :

Email: Yan.xu@cchmc.org