Singleplex real-time RT-PCR assay of porcine epidemic diarrhea virus (PEDV) viral load in Pampanga, Philippines

9^th Global Veterinary Summit

November 16-17, 2017 | Las Vegas, USA

Jacob Anderson C Sanchez, Tambalo, Gerald O Alili, Rubigilda P and Domingo Clarissa Yvonne

Pampanga State Agricultural University, Pampanga
AFP Medical Center, Philippines
Central Luzon State University, Philippines

Posters & Accepted Abstracts: J Vet Sci Technol

Abstract :

Pampanga is one of the main sources of pork used for food processing and culinary in Central Luzon, Philippines. Recently, farm owners reported mortality among pigs due to a diarrhea which might be Porcine Epidemic Diarrhea Virus (PEDV). Recent qualitative studies showed the presence or absence of the virus. Here, we aimed to conduct an absolute quantification of the viral load in fecal samples from suspected sucklings and weanlings using a singleplex real-time reverse transcription polymerase chain reaction (qRT-PCR). We report that the average viral load at the time of collection are farm s sucklings, 1.7X102 copy numbers (cn), farm s weanlings, 21X1010 cn, farm r sucklings, 9.0X107 cn, farm r weanlings, 1.3X106 cn. PEDV was detected at a sensitivity range of 1.89 X 10-7 up to 1.89 X 101 cn. The viral load reference index was as low as 4.44 X 10-21 cn and as high as 9.82 X 1016 cn. Given the positive result for PEDV and 100% mortality among the collected samples, it can be said that PEDV was present in the two farms of the province. These data may serve as a research-based reference for the Department of Agriculture in crafting and implementing policies for the swine industry. Furthermore, it will encourage the industry to prepare management and preventive measures against the virus.