Spectrophometric methods for Simultaneous determination of Valsartan and Nebivolol hydrochloride in combined tablet dosage form

2^nd International Conference and Exhibition on Pharmaceutical Regulatory Affairs

November 23-24, 2012 Hyderabad International Convention Centre, India

Shradhanjali Singh and Anil Kumar Singh

Accepted Abstracts: Pharmaceut Reg Affairs

Abstract :

Tablet dosage form accounts for approximately 50% of all dosage form on the market. The majority of tablets are used in the oral administration of drugs, which is the most convenient mode for drug administration. Validation of an analytical procedure is performed in order to demonstrate that the procedure is intended for its use. Analytical method development plays important role in discovery and manufacture of dosage form. Official test methods are used by Quality Control laboratories to ensure the identity, purity, potency and performance of drug product. Spectrophotometric method proved to be simple, fast, & precise, and thus can be used in routine work for the analysis of multicomponent formulation. The tablet dosage form selected is combination of Nebivolol hydrochloride and Valsartan. Two simple, accurate and precise spectrophotometric methods for the simultaneous determination of Valsartan (VAL) and Nebivolol hydrochloride (NEBI) in combined dosage form have been developed and validated. First spectroscopic method employs simultaneous equation method using 247.8 and 280.2 nm as two wavelengths for absorbance measurement using methanol and 0.1N HCl as the solvents. Beer's law is obeyed in the concentration range of 20 ? 100 μg/mL and 2.5 ? 40 μg/mL for VAL and NEBI, respectively. Second method is based on the determination of ?Area under Curve (AUC)? within the wavelength range of 278.2 ? 282.2 nm and 245.8 ? 249.8 nm. Molar absorptivities, Limit of Detection (LOD) and Limit of Quantitation (LOQ) were calculated. Both methods have been successfully applied for the analysis of these drugs in a pharmaceutical formulation. Results of analysis were validated statistically and by recovery studies.

Biography :

Shradhanjali Singh has completed her M Pharm at the age of 24 years from AISSMS College of Pharmacy, Pune University, Maharashtra in 2008. She is working as an assistant professor in United Institute of Pharmacy since last 1.5 years. She has 4 to 5 papers published in reputed journals. She has a life membership of APTI.