Steroidomics: Method development and applications

5^th International Conference and Exhibition on Metabolomics

May 16-18, 2016 Osaka, Japan

Nilesh W Gaikwad

University of California, USA

Posters & Accepted Abstracts: Metabolomics

Abstract :

Although steroids play a broad and vital role in human physiology, they are also implicated in the development and/or progression of many cancers viz. breast, ovarian, prostate, endometrial, liver, colon, etc. as well as in neurodegenerative diseases, cardiovascular disease and obesity. Present evidence shows the involvement of steroids; such as estrogens, testosterone, bile acids, oxysterols, etc. and their metabolites in disease processes. Hence, measurement of steroids in biological samples is essential to monitor human health. Currently, there is radioimmunoassay, gas chromatography�mass spectrometry (GC/MS), and liquid chromatography�mass spectrometry (LC-MS) methods developed for steroid measurements in biological samples. However, these methods require elaborate sample preparation procedures and have concerns(s) related to reproducibility, dynamic range, time, costs, and most importantly the total coverage of steroids. Also currently, there is no method available for comprehensive steroid metabolome profiling that includes androgens, corticosteroids, progestrogens, estrogens, estrogen metabolites, bile acids, oxy sterols, neurosteroids, steroid conjugates and steroid-DNA adducts. Here, I present a global steroid metabolic profiling method based on liquid�liquid extraction (LLE) followed by ultra performance liquid chromatography (UPLC)-tandem mass spectrometry (MS/MS) for simultaneous measurement of over 100 indigenous steroids from all classes. The method was successfully applied to determine steroid hormone levels in various settings. Development of steroidomics platform and recent studies involving steroid metabolic pathways and their association with various diseases will be discussed.

Biography :

Email: ngaikwad@ucdavis.edu