Study of protector effect of chemlal olive leaves extract on nephropathy induced by mercury in mouse

6^th World Congress on Medicinal Chemistry and Drug Design

June 07-08, 2017 Milan, Italy

Dekdouk Nadia, Ameddah Souad, Menad Ahmed, De Tommasi Nunziatina, Malafronte Nicola and Mekkio Ratiba

Universit�© des Fr�¨res Mentouri Constantine, Algeria
University of Salerno, Italy

Posters & Accepted Abstracts: Med Chem (Los Angeles)

Abstract :

Olea europaea L. is a typical fruit-tree widely cultivated in the Mediterranean area, belonging to Oleaceae family. The olive leaves contain high concentrations of phenolic compounds. The objective of this study was to evaluate the protector effect of chemlal olive leaves extract on nephropathy induced by mercury in mouse. To evaluate this, five experimental groups (n=6) are used. Group 1 (n=6) only vehicle (0.9% NaCl) in equal volume to mercury treated animals, was given to serve as control, Group 2 (n=6), the animals were treated with HgCl2 5 mg/k.bw in 0.9% NaCl (i.p.). Group 3 (n=6), the animals were administrated with olive leaves extract (OLE) (200 mg/kg b.w.) orally for 10 days. Group 4 (n=6), the animals were administrated sodium selenite (0.1 mg/kg) orally for 10 day before mercuric chloride (5 mg/kg b.w.), in Group 5, the animals were treated with (OLE) (200 mg/kg b.w.) orally for 10 days before mercuric chloride (5 mg/kg b.w.). In this study, treatment with Hg significantly increased serum urea and creatinine levels, myeloperoxidase (MPO) activity, NO and the malondialdehyde (MDA) level, as indicator of lipid peroxidation. The content of LDH, GSH and the activities of kidney antioxidant enzymes: Superoxide dismutase (SOD), catalase (CAT), GSH reductase and transferase were decreased. Selenium or oral administration of (OLE) before mercury treatment significantly lowered the serum levels of urea and creatinine. Furthermore, the content of MDA in kidneys decreased significantly. In addition, this extract increased LDH, the content of GSH, the antioxidant activities and decreased MPO activity and NO production. These results show that leaf extract possessed a protector effects, antioxidant and anti-inflammatory actions and would seem to be applicable in both the health and medical food.

Biography :

Dekdouk Nadia has completed her PhD in 2016 from animal biology department, faculty of science, Constantine 1 University and Post-doctoral studies from Constantine 1 University. In collaboration with an Italian research group from University of Salerno, department of pharmaceutical and biomedical sciences, she has published a paper in Evidence-Based Complementary and Alternative Medicine titled Phenolic compounds from Olea europaea L. possess antioxidant activity and inhibit carbohydrate metabolizing enzymes in vitro. The focus of her researches is the investigation of the hepatotoxic, nephrotoxic of some anticancer drugs and the interaction with Olea europaea phenolic compounds on rat.

Email: pharmacologie95@gmail.com