J.C. Lepivert, E. Desnouveaub, V. Casoli, M. Cario
INSERM 1035, France
Hospital Center University De Bordeaux, France
National Reference Center for Rare Skin Disease, France
Posters & Accepted Abstracts: J Tissue Sci Eng
Authors develop a biological dressing designed for patients presenting chronical wound, burn injury or congenital
melanocytic naevus. The reconstructed skin is produced under Good Laboratory Practices (GLP). Subsequently,
application of Autologous Pigmented Skin Dressing (APSD) on immunodeficient mouse model demonstrates its
harmlessness and functionality with the required safety controls. Keratinocytes, melanocytes and fibroblasts were
extracted from a patientâ??s biopsy and multiplied.
On top of the collagen matrix, fibroblasts were seeded to remodel collagen and secondly, keratinocytes and
melanocytes were seeded to produce the epidermal layer. APSD was produced in 3 to 5 weeks. From July 2018 to July
2019, 4 groups of 7 mice were implanted. For each group, 6 mice were treated with Test Item and one mouse with
collagen matrix alone as control. Defects of 6 cm² on dorsum of mice were done and covered with APSD or matrix
alone. Wound healing, clinical behavior, adverse events, tumor development and mortality signs were checked every
day. Groups 1, 3 and 4 healed well. Follow up demonstrated a good integration of APSD with minor retraction and
a diffuse pigmentation. Group 2 had skin retraction that increased during weeks. In the control group, wounds did
not heal and a complete skin retraction was present.
Bioengineered APSD demonstrated enthusiastic results regarding wound healing. It can be easily handled and
shipped.
APSD groups healed well except one group for which the quality of cells was initially worse as compared to cells for
the 3 other groups. A clinical trial is planned by mid-2020.
Journal of Tissue Science and Engineering received 807 citations as per Google Scholar report
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