Savita Sonawane, Mangesh Bhalekar and Shamkant Shimpi
Posters: Pharmaceut Reg Affairs
The aim of this study was to improve the mucoadhesive potential of xyloglucan polymer by the covalent attachment of cysteine as thiol moiety. The parent polymer xyloglucan was chemically first modified to carboxymethyl-xyloglucan intermediate then this was further converted to xyloglucan-cysteine conjugate by introducing sulphydryl bearing compound L-cysteine HCl in presence of EDAC as coupling agent. Different batches of conjugates were prepared at varying reaction pH (2-6) and evaluated for optimum thiol incorporation, disulphide group content, swelling behavior, rheological properties and mucoadhesive properties. The obtained conjugates characterized in vitro by quantification of immobilized thiol groups; showed maximum thiol incorporation on xyloglucan (8.63 ± 0.14 %) at pH 5. The disulphide group content was found maximum (3.54 ± 0.12 %) at pH 6. The % swelling index at end of 4 hr was 83.87 for xyloglucan and was found to decrease in thiolated derivatives, it was least for TH2 (76.93 %) and increased slightly till TH5 (82.78 %) and decreased further in TH6 (79.98 %). Mucoadhesion studies revealed that improvement in mucoadhesion of conjugate was three times compared to the unmodified polymer. The viscosity of thiomer was more than that of xyloglucan because of formation of disulphide bonds.
Ms. Savita Sonawane has completed her B. Pharm & M. Pharm from Pune University. She is now pursuing her Ph.D. from JNTU, Hyderabad. She is having 2 yrs of an industrial & 5 yrs of an academics experience. She has published paper in reputed journals and also presented posters in various conferences.
Pharmaceutical Regulatory Affairs: Open Access received 533 citations as per Google Scholar report