Systems biology approach on the elucidation of the response of Medicago truncatula plants towards salinity stress

3^rd International Conference and Exhibition on Metabolomics & Systems Biology

March 24-26, 2014 Hilton San Antonio Airport, San Antonio, USA

Vasileios Fotopoulos

Accepted Abstracts: Metabolomics

Abstract :

Salt stress is one of the most important factors limiting plant productivity, with salinity affecting plant physiology and metabolism at multiple levels. The aim of this study was to explore, elucidate and decipher the role of antioxidant and salt tolerance mechanisms in the model legume Medicago truncatula . For this reason, three ecotypes of M. truncatula showing differential response to salinity were used: Jemalong A17 (moderate response), TN6.18 (sensitive to salinity) and TN1.11 (tolerant to salinity). Cellular damage levels were monitored in roots and leaves after 48 h of salt stress application with 200 mM NaCl by measuring lipid peroxidation levels, as well as nitric oxide and hydrogen peroxide content, further supported by leaf stomatal conductance and chlorophyll fluorescence readings. The salt-tolerant genotype TN1.11 displayed the lowest cellular damage and ROS/RNS content, while the salt-sensitive TN6.18 was affected the greatest. Transcriptional profiling using microarray analysis of salt-stressed M. truncatula displayed differential gene expression that was both genotype and tissue-dependent. A large number of regulatory genes (associated or not previously linked with salinity stress) from a variety of biochemical pathways showed a significant induction/suppression pattern. Furthermore, metabolite profiling of M. truncatula plants was employed to analyse the effect of salt stress in the accumulation of key metabolites and their interrelationships, leading to exclusive insights into the plants? metabolic networks which however appear to be genotype- and not tissue- dependent. This global approach (with the addition of currently performed proteomics analysis) will hopefully contribute in gaining new insights into the cellular response to salt stress in M. truncatula plants.

Biography :

Vasileios Fotopoulos completed his Ph.D. at the age of 25 years from Southampton University, UK and postdoctoral studies from Aristotle University of Thessaloniki and CERTH, Greece. He is the leader of the Plant Stress Physiology Group at the Cyprus University of Technology in Limassol, Cyprus. He has published 26 papers in reputed journals as well as 4 book chapters and is currently serving as an editorial board member of 5 international plant sciences journals.