Technological platforms for stem cell lines characterization and control: The importance to evaluate cell line variations

2^nd International Conference on Cytopathology & Histopathology

August 10-12, 2016 Las Vegas, USA

Pasquale De Blasio

Integrated Systems Engineering S.R.L, Italy

Posters & Accepted Abstracts: J Cytol Histol

Abstract :

The stability of pluripotent stem cells depends on intrinsic (genetic background, mitochondrial fitness and selective pressure) and extrinsic factors (extracellular matrix, medium composition, passaging techniques, freezing and thawing techniques and oxidative stress) these can determine microdeletions, point mutations, genetic instability, karyotype abnormalities, epigenetic changes which ultimately lead to altered behavior including differentiation and tumorigenic potential. There is a great necessity to qualify the stem cells in order to fulfill results reproducibility. This can be achieved by creating technological platforms to evaluate systematically cell line variations, their characterization and behavior control.

Biography :

Email: pasquale.deblasio@isenet.it