The effect of dilution rate and insemination protocol on fertilizing capacity of frozen-thawed Arabian horse sperm

3^rd International Veterinary Congress

August 18-20, 2016 London, UK

Magdi Mohamed Waheed, K O Al-Khaldi and N Pratap

King Faisal University, Saudi Arabia
Cairo University, Egypt
Royal Oman Police, Oman
Royal Court Affairs, Oman

Posters & Accepted Abstracts: J Vet Sci Technol

Abstract :

Forty-eight ejaculates were obtained from four Arab stallions to study the impact of three extenders (INRA Freeze�®, Tris egg-yolk and E-Z mixin) on the fertilizing capacity of frozen-thawed sperm. Semen samples were diluted using the extenders with a dilution rate of 1:1 and 1:2 and cryopreserved in 0.5 ml plastic straws. Frozen straws were thawed either at 37 �°C for 30 sec or 75 �°C for 7 sec. Results revealed that the percentages of progressive sperm motility, live sperm and abnormalities and characters of sperm motility like path velocity (VAP, �¼m/s), straight line velocity (VSL, �¼m/s), point to point velocity (VCL, �¼m/s) and lateral head displacement (ALH, �¼m) were significantly (P<0.01) better in the INRA Freeze�® extender after thawing at 37 �°C with dilution rate 1:1 and 1:2. Sperm progressive motility after thawing at 75 �°C were significantly (P<0.01) higher by using INRA freeze�® and E-Z mixin extenders at rates of dilution 1:1 and 1:2. The conception rate in forty Arab mares using 300 million forward motile sperm per insemination was significantly (P<0.01) higher by using INRA freeze�® extender that thawed at 37 �°C at dilution rate of 1:1 (50%) than 1:2 (20%). The use of two times inseminations gave significantly (P<0.01) better results (50%) than the use of one time insemination (28.57%). In conclusion, the fertilizing capacity of the frozen-thawed Arabian horse sperm was the best by using INRA freeze�® extender in a dilution rate of 1:1 and two times inseminations.

Biography :

Email: mmwaheed@kfu.edu.sa