The effect of the small amount of formalin in the SurePath liquid when establishing protocols for immunocytochemistry

International Conference on Cytopathology

August 31-September 02, 2015 Toronto, Canada

Torill Sauer

Akershus University Hospital, Norway

Posters-Accepted Abstracts: J Cytol Histol

Abstract :

Objective: To investigate the effect of the small amount of formaldehyde in the SurePath liquid (< 0.2 %) when establishing a routine protocol for immunocytochemistry. Materials & Methods: Rest material from unfixed effusions containing either carcinoma cells or a fair number of mesothelial cells. The cells were transferred to Surepath and left in the liquid for 1, 5 and 12 days before immunostaining. Day 0 equaled a staining protocol not using Surepath for fixation. CK7 was used as a test marker because it was easy to find suitable rest effusion material. Immunostaining was done at several time points and with varying protocols for pretreatment and incubation of CK7 AB. Adding or omitting neutral buffred formalin (NBF) was also recorded. The cytoplasmic staining intensity of carcinoma cells and/or mesothelial cells was evaluated. Results & Conclusions: The small amount of formaldehyde in SurePath influences the immuno reactivity of cells in the liquid. Reduced staining intensity may be seen within 5 days of storage in the SurePath liquid. HRT is essential and the pretreatment must reflect the routine storage time of cells in Surepath liquid before eventual immunostaining.

Biography :

Torill Sauer completed her PhD in 1999 at the University of Oslo. She is a Professor of pathology at the University of Oslo since 2005. She has published about 80 papers in reputed journals.

Email: torill.sauer@medisin.uio.no