The functional state of the chaperone Hsp27 as a molecular marker of lymph node metastases of breast cancer

International Conference and Exhibition on Molecular Medicine and Diagnostics

August 24-26, 2015 London, UK

Evgeniya V Kaigorodova

Posters-Accepted Abstracts: J Mol Genet Med

Abstract :

Background: Heat shock protein 27 kDa (Hsp27) is a chaperone of the sHsp (small heat shock protein). The common functions
of sHsps are chaperone activity, inhibition of apoptosis, regulation of cell development, and cell differentiation, take part in signal
transduction.
Objective: To study the intracellular localization of phosphorylated features and non-phosphorylated forms of Hsp27in primary
breast cancer cells and to evaluate their relationship with regional lymphatic metastasis.
Materials & Methods: Tumor biopsies of breast tissue were collected from 100 patients with a confirmed diagnosis of invasive
carcinoma, nonspecific type, between the ages of 31-80 years. Immunohistochemistry was used to determine the intracellular
localization of phosphorylated and non-phosphorylated forms of Hsp27.
Results: The result of this study showed that biopsies from patients with lymph node metastasis exhibited significantly higher levels
of phosphorylated forms of Hsp27 in the nucleus and cytoplasm compared with the group without lymph node metastasis. Analysis
showed that the expression of phosphorylated forms of the chaperone Hsp27 correlates with the amount and percentage of lymph
node metastases affected.
Conclusion: The nuclear expression of phosphorylated and non-phosphorylated forms of the chaperone Hsp27 is a marker of tumor
cells associated with lymphatic metastasis of breast cancer.

Biography :

Evgeniya V Kaigorodova, PhD, MD is a physician of clinical laboratory diagnostics of the highest category. She is a leading researcher, Department of Pathological Anatomy
and Cytology, Tomsk Cancer Research Institute (2012-present); leading researcher Laboratory of Translational Cell and Molecular Biomedicine, Tomsk State University
(2014-present). She completed her PhD from Siberian State Medical University in 2008 and Postdoctoral studies from Siberian State Medical University. She is a Professor
of the Department of Biochemistry and molecular biology of the Siberian State Medical University (2014-present). She has published more than 50 papers in reputed
journals.