The great potential of phenolic compounds isolated from Limonium densiflorum to quench and protect human cell against free radicals

6^th World Congress on Medicinal Chemistry and Drug Design

June 07-08, 2017 Milan, Italy

Faten Medini, Manel oueslati, Jean Legault and Riadh Ksouri

Biotechnology Center of Borj Cedria, Tunisia
University of Quebec in Chicoutimi, Canada

Posters & Accepted Abstracts: Med Chem (Los Angeles)

Abstract :

The diversity of plant-based systems has provided an enormous number of lead compounds in healthcare. The crucial factor for the ultimate success of an investigation on bioactive plant constituents is thus the selection of plant materials and the appropriate extraction and purification process of the active compounds. Halophytes plants, living in extreme environments dealing with frequent changes in the salinity level, are used to treat various diseases and aging processes. Among of them, the genus Limonium is known in the traditional medicine. The chemical composition of the ethanolic shoot extract of L. densiflorum showed excellent radical with scavenging and antioxidant properties. Furthermore, it represents a rich and growing source of natural target molecules, such as phenolic compounds. In order to isolate the active compounds, an in vitro fractionation was undertaken by preparative chromatographic techniques. On the basis of nuclear magnetic resonance techniques, the structure of the isolated compounds was determined as gallic acid, epigallocatechin gallate, quercitrin, dihydrokaempferol, pinoresinol, N-trans-ferulolyltyramine and (myricetin 3-O-a-rhamnopyranoside and myricetin 3-O-L-arabinofuranoside). All isolated molecules were evaluated for their capacities to inhibit ROS formation on fibroblast cell line (WS-1) by the 2�´,7�´-dichlorofluorescein assay. Results showed that all compounds tested were found to reduce ROS formation at various doses unless the phenol amide trans-N-ferulolyl tyramine (IC50>50 �¼g/ml). Epigallocatechin gallate followed by gallic acid and the mixture of myricetin 3-O-�±-rhamnopyranoside+myricetin 3-O-Larabinofuranoside, showed the highest antioxidant activity with IC50 values of 0.92, 1.22 and 1.5 �¼g/ml, respectively.

Biography :

Email: medinifaten@yahoo.fr