The use of quantitative real-time polymerase chain reaction (qRT-PCR) technologies to screen for the presence of total bacteria in a liquid sample

6^th International Conference and Exhibtion on Pharmaceutical Regulatory Affairs and IPR

September 29-30, 2016 Orlando, USA

Andy Moreno

HSG/AME Certified Laboratories LLC, USA

Accepted Abstracts: Pharmaceut Reg Affairs

Abstract :

The purpose of this paper is to report the results of a novel test and release research study substantiating the use of quantitative, real-time polymerase chain reaction (qRT-PCR) technologies for the detection of human pathogens in fresh produce. Traditional culture-based testing for the presence of these pathogens requires from 2 to 14 days. The uniqueness of this presentation is the application of qRT-PCR technologies to the surveillance screening fresh produce for pathogens, such as E. coli, Salmonella, and Listeria. With the application of qRT-PCR technologies, decision-makers can know of the presence of these pathogens within one (1) one hour. The research was to gather quantitative and qualitative data in a mixed method research project to support the introduction of qRT-PCR technologies. The objective of the study was to substantiate the theory that the envisioned application of qRT-PCR technologies in a one (1) hour test and release practicable, industrial scheme is supportable and viable. The strengths and weaknesses of the study�s methods and designs were deliberated. The justifications to support the research why the engaged methods and designs were the most appropriate for this research were discussed. Alternative methods/designs were also reviewed and explained why they were less desirable for the purposes of this study. The ultimate objective of the study was to corroborate all assertions with scholarly research and to provide policy makers with valid data which supports whether qRT-PCR technologies can be applied to practical test and release programs for fresh produce.

Biography :

Email: andy.moreno@ame-qpcr.com