TIM-3 gene polymorphisms and risk of multiple sclerosis

2^nd International Conference on Neuroimmunology & Therapeutics

December 01-02, 2016 Atlanta, USA

Maryam Izad and Esmat Yaghoobi

Tehran University of Medical Sciences, Iran

Posters & Accepted Abstracts: J Neurol Disord

Abstract :

Multiple sclerosis (MS) is an autoimmune disease of the central nervous system (CNS). It would be caused by auto reactive T-cells directed against myelin antigens. T-cell immunoglobulin mucin-3 (TIM-3) is a negative regulator glycoprotein expressed by a range of immune cells, including, Th1 cells, activated CD8+ T-cells and in a lower level on Th17 cells. TIM-3 might have a key role in the autoimmune diseases by interacting with TIM-3 ligand to regulate T-cells responses. A defect in TIM-3 regulation has been shown in multiple sclerosis patients. In humans, several single nucleotide polymorphisms (SNPs) have been identified in the TIM-3 gene and are associated with inflammatory diseases. The present study analyzes the association between TIM-3 -574A>C and -1516 C>A gene polymorphisms and susceptibility to MS. DNA samples from 102 patients and 102 healthy controls were genotyped using RFLP-PCR method. Analysis of the alleles and genotypes revealed a significant higher frequency of C/C and lower frequency of A/C genotypes for -574 locus of TIM-3 gene in MS patients (P=0.0002). We also found that C/C genotype for locus of -1516 increased in MS patients, while A/C genotype decreased (P=0.012). Allele C of -574C/C and -1516 C>A SNPs were also more frequent in MS patients (P=0.036 and 0.0027 respectively). These findings suggest that -574 A>C and -1516 C>A polymorphisms in the promoter region of TIM3 gene may affect the disease susceptibility.

Biography :

Email: izadm@tums.ac.ir