Transcriptomic profiling reveals molecular aspects of nitrogen oxide-induced adventitious rooting in mung bean seedlings

9^th International Conference on Genomics & Pharmacogenomics

June 15-16, 2017 London, UK

Shi-Weng Li, Guo-Jin Zhang and Yan Leng

Lanzhou Jiaotong University, China

Posters & Accepted Abstracts: J Mol Genet Med

Abstract :

Recent studies showed that nitrogen oxide (NO) strongly promoted adventitious rooting in plants. To gain further insight into the molecular mechanisms underlying this process, the transcriptome analysis was performed using RNA-Seq and qRT-PCR technologies. The RNA-Seq data showed that NO donor sodium nitroprusside treatment significantly enhanced gene expression at the root induction stage but reduced gene expression at the root initiation stage compared with the water and IBA treatments. GO enrichment analysis indicated that oxidoreductase activity was the common GO subcategory that was significantly regulated in all the samples; and microtubule-based process, nitrogen compound response, cell cycle, and hydrolase activity were the most highly up-regulated GOs at the root induction stage while response to stress, response to chemical stimulus, nitrate and sulfur compound transmembrane transporter activity, and cell wall biogenesis were the most highly up-regulated GOs at the root initiation stage by NO. KEGG pathway enrichment showed that cell cycle, metabolism of terpenoids, lipid metabolism, protein processing, energy metabolism, phenylalanine metabolism, and xenobiotics biodegradation were the most highly up-regulated pathways at both the root induction and initiation stages by NO. The analysis of the most highly regulated genes (RPKM�10 and fold change�2) indicated that NO significantly regulated the genes associated with nitrogen compound response, stress response, oxidative stress response, cell wall modification, signal transduction, protein processing, secondary metabolism, metabolic processes, and transcription factors, as well as plant hormone signaling, including auxin, ethylene, cytokinin, gibberellin, and abscisic acid pathways. These results strengthen the current understanding of NO-induced adventitious rooting and the molecular traits of NO in plants.

Biography :

Email: lishweng@mail.lzjtu.cn