Transgenic mice with high endogenous omega-3 fatty acids are protected from ischemiareperfusion- induced acute kidney injury

2^nd International Conference on Nephrology & Therapeutics

July 29-30, 2013 Embassy Suites Las Vegas, NV, USA

Hyoin Jeon, Heera Yang, Dongwook Seo, Kyu Lim2, Won-min Hwang and Jaeku Kang

Posters: J Nephrol Therapeutic

Abstract :

S everal studies have shown that omega-3 polyunsaturated fatty acids (PUFAs) diet can reduce kidney dysfunction following ischemic injury. fat-1 transgenic mice produce abundant omega-3 PUFAs, resulting in balanced omega-6 : omega-3 ratio in comparison to wild type (WT) mice. Therefore, the purpose of this study was to determine whether omega-3 PUFAs are protective in AKI caused by ischemic injury using fat-1 transgenic mice. To induce AKI, mice were subjected to renal ischemia-reperfusion injury (IRI). Animals were sacrificed at 24 hr and 72 hr of post-reperfusion. After that, renal function and severity of renal injury were estimated. BUN (blood urea nitrogen) and serum creatinine levels were decreased in fat-1 group at 24 hr post-IRI, although the difference was not statistically significant. The levels were significantly decreased in fat-1 group compared to WT group at 72 hr post-IRI. The difference in Kim-1 expression was not statistically significant due to intragroup variation, but the fold-increase in Kim-1 expression in fat-1 group was less than that in WT group. Relatively more lesions were observed on fat-1 group than on WT group at 24 hr post-IRI. However, at 72 hr post-IRI, more lesions were observed in WT than in the fat-1 group. Neutrophil infiltration was reduced at 72 hr in the fat-1 group as compared to the WT group. This study demonstrates that omega-3 PUFAs exert a protective effect on ischemia-induced AKI. Long-term and high-dose omega-3 supplements may preserve renal function and facilitate renal recovery following AKI