Triggering of suicidal erythrocyte death by uremic toxin indoxyl sulfate

8^th World Nephrology Conference

August 15-16, 2016 Sao Paulo, Brazil

Mohamed Siyab Eldin Elsadig Ahmed

Central Veterinary Research Laboratory, UAE

Scientific Tracks Abstracts: J Nephrol Ther

Abstract :

Introduction: Anemia in end stage renal disease is attributed to impaired erythrocyte formation due to erythropoietin and iron deficiency. On the other hand, end stage renal disease enhances eryptosis, the suicidal erythrocyte death characterized by cell shrinkage and phosphatidyl serine-exposure at the erythrocyte surface. Eryptosis may be triggered by increase of cytosolic Ca2+- activity ([Ca2+]i) and by ceramide, which sensitizes erythrocytes to ([Ca2+]i). Mechanisms triggering eryptosis in end stage renal disease remained enigmatic. The present study explored the effect of indoxyl sulfate, an uremic toxin accumulated in blood of patients with chronic kidney disease. Methods: In this study, cell volume was estimated from forward scatter, phosphatidylserine-exposure from annexin V binding, ceramide abundance by specific antibodies, hemolysis from hemoglobin release, and [Ca2+]i from Fluo3-fluorescence. Results: Our results showed that a 48 hours exposure to indoxyl sulfate significantly increased [Ca2+]i (â�¥300 �¼M), significantly decreased forward scatter (â�¥300 �¼M) and significantly increased annexin-V-binding (â�¥50 �¼M). Indoxyl sulfate (150 �¼M) induced annexin-V-binding was virtually abolished in the nominal absence of extracellular Ca2+. Indoxyl sulfate (150 �¼M) further enhanced ceramide abundance. Conclusion: Finally we concluded that Indoxyl sulfate stimulates suicidal erythrocyte death or eryptosis, an effect in large part due to stimulation of extracellular Ca2+ entry with subsequent stimulation of cell shrinkage and cell membrane scrambling.

Biography :

Mohamed Siyab Eldin Elsadig Ahmed has completed his Doctorate from University of Tuebingen. He is the Head of the Department of Molecular Biology. He has published about 10 papers in reputed journals. He is also an Editorial and Advisory Board Member of JBRC.

Email: m_sea_74@daad-alumni.de