Ultra-trace analysis of D and L aspartic acid applying one-by-one approach on a dual imprinted electrochemical sensor

7^th World Congress on Biopolymers and Polymer Chemistry

June 04-06, 2018 Osaka, Japan

Swadha Jaiswal, Bhim Bali Prasad and Kislay Singh

Banaras Hindu University, India

Posters & Accepted Abstracts: J Material Sci Eng

Abstract :

An enantiomeric pair imprinted polymeric film with embedded functionalized multiwall carbon nanotubes was developed following the â��surface grafting fromâ�� approach onto a gold nanoparticle decorated pencil graphite electrode. Double imprinting of chiral molecules in a single polymer motif, as a sensory platform for the one-by-one evaluation of individual components of a racemic mixture, is a challenging task. The underlying state-of-art proposed for this purpose is novel and if this works well, any two simple molecules could be used as templates. In this work, a pencil graphite electrode was first dipped for the overnight in the aqueous suspension of gold nanoparticles. The electrode was then subjected to spin coating with a prepolymerization mixture consisting a monomer (N-acryloyl-pyrrolidine-2,5-dione), templates (D and L aspartic acid), a crosslinker (ethylene glycol dimethacrylate) in the presence of an initiator (�±,�±â��-azoisobutyronitrile). As aspartic acid isomers have been found to be electro-inactive, their evaluation was feasible indirectly with the help of a potassium ferricyanide probe. The quantitation ability of the proposed sensor with differential pulse anodic stripping voltammetric transduction was found to be in the tune of 4.08 ng mL-1 (S/N=3) for both the enantiomers in the real samples, which reportedly manifest several chronic diseases at their stringent limits. swadhu.98@gmail.com