Unnatural fluoro-oxindole alkaloids produced by Uncaria guianensis plantlets

European Chemistry Congress

June 16-18, 2016 Rome, Italy

Adriana A Lopes, Bruno Musquiari, Suzelei de C Franca and Ana Maria S Pereira

Universidade de Ribeir���£o Preto, Brazil

Posters & Accepted Abstracts: Chem Sci J

Abstract :

Natural products and their analogues have been sources of numerous important therapeutic agents. The medicinal plant U. guianensis (Rubiaceae) cultured in vitro produce four oxindole alkaloids that displays anti-tumoral activity. Natural products can be modified by several approaches, one of which is precursor-directed biosynthesis (PDB). Thus, the aim of this work was apply precursor-directed biosynthesis approach to obtain oxindole alkaloids analogues using in vitro Uncaria guianensis. Plantletes were cultivated into culture medium supplemented with 1mM of 6-fluoro-tryptamine, the indol precursor of alkaloids biosynthesis. U. guianensis explants were maintained at 25���±2���°C, 55-60% relative humidity under the same photoperiod and light intensity. After 30 days, a methanolic extract from U. guianensis was obtained and analysed by HPLCDAD analytical procedure. The chromatogram showed four natural alkaloids (mitraphylline, isomitraphylline, rhynchophylline and isorhynchophylline and four additional peaks. Semi-preparative HPLC allowed isolation and purification of these four oxindole alkaloids analogues and the identity of the peaks was confirmed from high-resolution MS data (HRESIMS/MS in positive mode). All data confirmed that Uncaria guianensis produced fluoro-oxindole alkaloids analogues.

Biography :

Adriana A Lopes is from Universidade de Ribeirão Preto, Brazil.
Email:adrianalps@yahoo.com.br