X-STR and SNP and mtDNA analysis in maternity testing when the false mother can’t be excluded by 46 STRs genotyping

2^nd International Conference on Forensic Research and Technology

October 07-09, 2013 Hampton Inn Tropicana, Las Vegas, NV, USA

Li Li

Accepted Abstracts: J Forensic Res

Abstract :

To reach an accurate conclusion, X-STR, SNP and mtDNA analysis was applied to an extreme maternity case where the false mother and the child have at least one allele shared at autosomal 46 STR loci. Method: 19 autosomal STR loci were amplified using the AmpFlSTR® SinofilerTM kit and PowerPlex®16 System. An additional 27 autosomal STR loci were analyzed using two domestic kits AGCU 21+1 and STRtyper-10G. Further testing of 24 X-STR loci, 40 SNP loci and mtDNA was carried out. Particularly, 24 X-STR loci were amplified using Mentype® Argus X-8 kit and an in-house kit Xplex-16. mtDNA HV1 and HV2 were amplified using primer pairs L16047/H16464, L29/H408 respectively and the PCR products were sequenced. Additionally, SNP assay was carried out and mtDNA assay of base compositions was applied. Using PLEX-ID system, base compositions of 12 amplicons in HV1 derived from primers covering coordinates15924-16428 and base compositions of 12 amplicons in HV2 derived from primers covering coordinates 31-576 were determined. Results: The alleged mother and the boy shared at least one allele at all 46 tested autosomal STR loci. But, according to the profile data for 24 X-STR and 40 SNP markers, different genotypes at 13 X-STR loci and five SNP loci excluded maternity. Mitochondrial profiles also clearly exclude mother as a parent of the son because they have multiple differences. Conclusions: Different kinds of genetic markers needfully supplement the use of autosomal STR loci in case where the alleged parent is suspected to be related to the true parent.

Biography :

Li Li is a Professor of department of forensic Biology in Institute of Forensic Sciences, Ministry of Justice, China. Her responsibilities include forensic DNA analysis (typing of STR, X-STR, Y-STR, SNP, X-SNP and mtDNA) and testify in court. She processes thousands of cases including parentage and sibling testing every year.