SUBHASH SISTA

SUBHASH SISTA
Research Scholar National Institute of Standards and Technology (NIST), Gaithersburg, Maryland, 923 Clopper Road, Apt T1, MD 20878 Phone +1 (240) 406 2539 subhash.sista@nist.gov
USA

Biography

Dr.SUBHASH SISTA has Around 8 years of research experience with 3 years (Pre and Post Ph.D) of working in prestigious laboratories at the national and international level namely Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India as Project Assistant-II and as a Research Scholar at National Institute of Standards and Technology (NIST), Maryland, USA. • Worked on projects pertaining to Stem cell biology, Tissue engineering and regenerative medicine; Protein and enzyme engineering, RNA biology (qPCR). • Expertise in quantitative imaging and automated microscopy, flow cytometry, electron microscopy, protein purification and characterization and other biophysical techniques. • Excellent written and communication skills as evident by publications in international journals. • Experience as a peer reviewer for reviewing articles for several international journals. • Active lab manager for providing safety training, equipment training, risk assessment and hazard reviews pertaining to the lab activities. • Understand collaborative skills requirements, easily adaptable to be able to deal with changing circumstances, teamwork skills and ability to work in multi-disciplinary environment. • Ability to lead a team, to effectively work with a wide range of colleagues and take control of the project to deliver results in a timely and quality manner. • Skilled at developing SOPs and study related documents like protocols, management plans (monitoring, risk, safety etc), study manuals and guidelines. • Knowledge and expertise in principles and practice of current Good Manufacturing Practices (cGMP) and Good Laboratory Practices (GLP).

Research Interest

Human embryonic stem cell colonies, OsteoblastsIsolation of RNA from animal cells; Primer designing and Quantitative real time PCR (RT-PCR); Animal cell culture processes; Immuno- histochemistry; Histology (Sectioning and H&E staining of animal tissues); Microscopy (Processing of samples for Scanning Electron Microscope, Transmission Electron Microscope, Atomic Force Microscope, Laser Scanning Confocal and Multiphoton Microscope, Live Cell Imaging); FT-IR spectroscopy; Flow cytometry; 2-D electrophoresis; Contact angle measurements; Compression and Tensile tester for metals; Particle Size Analyzer; Hardness tester; X-ray Photo Electron Spectroscope for elemental composition analysis; Profilometer for roughness measurement.