This paper discusses the cleaning effect of shampooing care by using Adenosine Triphosphate (ATP) bioluminescence system. Shampooing care is an important nursing one for patients who are difficult to care oneself. It is necessary to perform effective and comfortable care. So, we need to evaluate these effects quantitatively and qualitatively. We measured ATP, blood pressure, heart rate, heart rate variability, peripheral skin temperature and Visual Analog Scale (VAS) before and after shampooing. When we analyzed ATP adhering to head as “contamination” to evaluate that effect quantitatively, it found that the contamination of the scalp after shampooing decreased by 31%, and that of the hair decreased by 22%. The correlation between ATP and VAS showed that the more ATP decreased, the more subjects felt refresh. The physiological index was stable, and it found that the shampoo method in this study had little load on the body. We were able to clarify the cleaning effect of shampooing using rapid and simple method. These results showed can be the basic data that evaluated the effect by shampooing comprehensively.